ASPECTOS ULTRAESTRUCTURALES DE ESTADIOS PREIMAGINALES E IMAGINALES DE CUATRO ESPECIES DE PHLEBOTOMINAE (DIPTERA: PSYCHODIDAE) DEL ESTADO TRUJILLO, VENEZUELA :: ULTRASTRUCTURAL ASPECTS OF PREIMAGINAL AND IMAGINAL STAGES OF FOUR PHLEBOTOMINAE SPECIES (DIPTERA: PSYCHODIDAE) OF TRUJILLO STATE, VENEZUELA)

Dalmiro Cazorla-Perfetti, Milagros Oviedo

Resumen


Ante el poder resolutivo limitado de la microscopía óptica, la Microscopía Electrónica de Barrido (MEB) aparece como una herramienta complementaria y útil para el estudio de la morfología de los integrantes de los Dípteros-psicodídeos de la subfamilia Phlebotominae. Por ello, en el presente trabajo se identificaron y describieron algunas características ultramorfológicas externas de huevos de Lutzomyia (Tricholateralis) gomezi; larvas estadio IV y pupas de Pintomyia (Pifanomyia) evansi; machos de Micropygomyia (Sauromyia) trinidadensis y hembras de Pi. (Pif.) youngi, obtenidas en condiciones de laboratorio a partir de hembras flebotominas capturadas en Trujillo, región andino-venezolana. El estudio ultramorfológico reveló que los huevos de Lu. (Trl.) gomezi poseen un patrón de esculpido exo-coriónico poligonal. Asimismo, se confirma que las larvas IV de Pi. (Pif.) evansi pueden colocarse en el Grupo cuatro de las especies flebotominas del Neotrópico que poseen antenas con segmento basal corto y el distal ovoide; mientras que la mayoría de las setas analizadas de sus pupas son simples y espiniformes, y las pre-alares largas, curvadas y delgadas con puntas afiladas. En varias regiones anatómicas de los imagos de Mi. (Sau.) trinidadensis y Pi. (Pif.) youngi se detectaron varios tipos de sensilas, incluyendo las escuamiformes, tricoideas, caéticas y microtriquias. Se discute la importancia de estos hallazgos ultraestructurales en el contexto de la taxonomía y sistema de quetotaxia de la subfamilia Phlebotominae, y de la posible función de las sensilas.

 

Palabras clave: Flebotominos, morfología, estadios inmaduros, adultos, Microscopía Electrónica de Barrido.

 

Abstract

 

Due to limitations in the resolution of optical microscopy, Scanning Electron Microscopy (SEM) appears to be considered as a useful complementary tool for the study on morphology of the Psychodid-Diptera subfamily Phlebotominae. Thus, in the present article some outer ultramorphological characteristics of laboratory reared eggs of Lutzomyia (Tricholateralis) gomezi; fourth instar larvae and puparium of Pintomyia (Pifanomyia) evansi; males of  Micropygomyia (Sauromyia) trinidadensis and females of Pi. (Pif.) youngi of Trujillo, Venezuelan andes region, were identified and described. The ultramorphological study revealed that the exo-chorionic sculpturing pattern of the eggs of Lu. (Trl.) gomezi are polygonal. Likewise, it is confirmed that the fourth instar larvae of Pi. (Pif.) evansi can be placed in Group 4 of the Neotropical phlebotomine which posses’ antennae with a short basal segment and an ovoid distal segment; whereas most of its analyzed puparium setae are short, smooth and spinniforms, and the pre-alar ones long, curved, thin and sharp. Various types of sensilla, including squamiform, trichoid, chaetic and microtrichia ones were detected on several anatomic parts of adults of Mi. (Sau.) trinidadensis and Pi. (Pif.) youngi. The importance of these ultrastructural findings to phlebotomine sandfly taxonomy and chaetotaxy system and possible function of sensilla, is discussed.

 

Key words: Sandflies, morphology, inmature stages, adults, Scanning Electron Microscopy.


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